European Funds KED - UMK - EU ESF
Academia Copernicana
Contactul. Lwowska 1, 87-100 Toruń
tel.: +48 56 611-44-44
e-mail: academia.copernicana@umk.pl

Generation and phenotypic analysis of transgenic lines of oleaginous microalga Nannochloropsis oceanica with overexpression and targeted mutations of lipid metabolism-related genes

 

Investigator

Title of the
project
Generation and phenotypic analysis of transgenic lines of
oleaginous microalga Nannochloropsis oceanica with overexpression and
targeted mutations of lipid metabolism-related genes
Primary and
secondary
scientific
disciplines
Molecular Biology and Biotechnology
Description
of the
project
In my Doctoral research I will be focusing towards deciphering and then
over-expressing lipid biosynthesis related genes thus enhancing the lipid
productivity in marine alga
Nannocloropsis sp.

 

Following are my main objectives and characteristics :
1) Deciphering the effect of lipid metabolism on the overall cell
metabolism by comparative Transcriptomic analysis between wild type and
previously developed DGTT 5 over-expressed mutants of
Nannochloropsis
sp.
2) Observing and characterizing the isoforms of DGAT encoding genes
getting over-expressed under different stress conditions by using RT-PCR
3) By using CRISPR-Cas9 technique selectively knocking off the TAG
encoding genes and their expression profiling by RT-PCR approach
4) Over-expressing selective DGAT encoding genes with knocking off the
TAG lipases
The project is conducted in cooperation with:
1) Prof. Zhi-Yan (Rock) Du, University of Hawaii at Manoa,
College of Tropical Agriculture and Human Resources,
Department of Molecular Biosciences & BioEngineering,
Honolulu, USA
2)Prof. Christoph Benning, Michigan State University, Plant
Biology Laboratories, East Lansing, USA
Principal supervisor: dr hab. Krzysztof Zienkiewicz, prof. UMK
Subsidiary supervisor: dr hab. Agnieszka Zienkiewicz, prof. UMK
Foreign scientific advisor:
Prof. Zhi-Yan (Rock) Du